http://hdl.handle.net/123456789/2233 البحث عن قناة بحث http://repository.ksu.edu.sa/jspui/simple-search http://hdl.handle.net/123456789/6837 العنوان: Are plasma derivatives sources for parvovirus B19 transmission?<br/><br/>المؤلفون: Baddour, Manal M.<br/><br/>ملخص: Parvovirus B19 is known to cause a spectrum of diseases ranging from mild illness tolife threatening disease. The icosahedral nonenveloped B19 virion is resistant toordinary physicochemical factors, including solvent-detergent treatment. Because ofits minute size, 20-25 nm, the pathogen is also relatively resistant to filtration. It canthus be transmitted through blood products to vulnerable recipients in which it causessevere disease. This study aimed at tracking the presence of parvovirus B19 inplasma products available in the local market in Saudi Arabia using nested PCR.Results showed the absence of detectable levels of parvovirus B19 DNA from thetested products. While this is reassuring, it should not entice the authorities to refrainfrom continuously monitoring blood products for the prese http://hdl.handle.net/123456789/6718 العنوان: Benign lichenoid keratoses with histologic features of mycosis fungoides: clinicopathologic description of a clinically significant histologic pattern<br/><br/>المؤلفون: Al-Hoqail, Ibrahim A; Crawford, Richard I<br/><br/>ملخص: Background: Benign lichenoid keratosis (BLK) is a well-known clinicopathologic entity and several histopathologic patterns have been described. Features mimicking mycosis fungoides (MF) in clinically typical BLKs have not yet been emphasized. The aim of this study was to confirm the occurrence of an MF-like pattern of BLK. Methods: A retrospective survey was conducted on cases diagnosed as BLK over a 9-month period in a regional dermatopathology service. Seven histologic parameters, previously confirmed as diagnostically suggestive of MF, were applied. Inclusion criteria were: three or more MF-related histologic features and a size less than 2 cm. The clinical features were reviewed. Results: Fifteen cases of MF-pattern BLK were identified. The number of MF-like parameters present in individual cases exceeded the inclusion criteria by variable amounts. Pautrier microabscesses and alignment of lymphocytes along the basal layer were the most frequent (14/15). The age of the patients ranged from 28 to 83 years, with a mean of 50. The size of the lesions ranged from 0.2 to 1.8cm, with a mean of 0.6cm. The upper trunk was the favored site. Most of the lesions had been removed because of suspicion of cutaneous malignancy; basal cell carcinoma was the most frequent clinical diagnosis. Conclusion: We describe an MF-like histologic pattern of BLK. Pathologists and dermatopathologists should be aware of this novel histologic pattern to facilitate distinction between the two disorders.<br/><br/>وصف: Department of Dermatology, King Khalid University Hospital and Faculty of Medicine at King Saud University http://hdl.handle.net/123456789/6166 العنوان: The potential anti-inflammatory effect of tetrahydrobiopterin administration in renal mass reduction-induced chronic renal failure in rats<br/><br/>المؤلفون: Korish, Aida A.; Arafah, Maha M.<br/><br/>ملخص: Objectives: To investigate the impact of tetrahydrobiopterin (BH4) supplementation on the markers of inflammation, and on the histological picture of the kidney in chronic renal failure C-reactive protein (CRF) induced in rats by subtotal nephrectomy (SNx). Methods: This study was performed at the Faculty of Medicine, King Saud University, Riyadh, Saudi Arabia during the period from December 2005 to January 2007. Chronic renal failure was induced by 5/6 SNx in 20 male Wister rats, and another 10 rats were sham operated by flank incision and served as controls. Ten SNx rats received 10mg kg-1 BH4 intraperitoneally daily for 4 weeks. Plasma C-reactive protein (CRP), interlukin-6 (IL-6), malondialdehyde (MDA), and kidney functions were measured in all rats. Histopathological examination of the kidney tissues was also performed. Results: Untreated CRF rats showed significant elevation of plasma CRP, IL-6, and MDA levels, and significant decrease in plasma albumin and total protein levels, tubuloglomerular fibrosis and, interstitial tubular infiltration with inflammatory cells in comparison with the sham-operated rats. Tetrahydrobiopterin treatment decreased CRP, IL-6, and MDA levels, and decreased tubuloglomerular fibrosis and interstitial inflammation in treated CRF rats. Conclusions: Supplementation with exogenous BH4 decreased markers of inflammation and protected the kidney against post-renal mass reduction histologic damage. Restoration of intracellular BH4 balance could normalize nitrous oxide production. Therefore, BH4 might be a promising strategy in attenuating inflammation in CRF. This may decrease endothelial dysfunction and limit the associated cardiovascular morbidity and mortality of this disease.<br/><br/>وصف: This article published in English, French and Arabic languages http://hdl.handle.net/123456789/2767 العنوان: Evaluation of 3 PCR techniques for detection of brucella DNA in peripheral human blood<br/><br/>المؤلفون: Baddour, Manal M; Alkhalifa, Dalal H<br/><br/>ملخص: Brucellosis is a widespread zoonosis transmittable to humans. Currently the diagnosis of this zoonosis is based on microbiological and serological laboratory tests. PCR has been used to detect DNA from Brucella. Different target genes, primer pairs, PCR techniques and extraction procedures have been previously published for Brucella detection. But only a few of these primers have been used in human samples, and only one study has been carried out to compare sensitivity between them. In the present study, 3 sets of primers and 3 different PCR protocols amplifying 3 different regions of the Brucella genome were compared for detection of Brucella DNA in a peripheral-blood PCR assay to conclude which is most suitable for the clinical diagnostic laboratory. These three pairs of primers amplify three different fragments included in: i) a gene encoding a 31-kDa B. abortus antigen (B4/B5), ii) a sequence 16S rRNA of B. abortus (F4/R2), and (iii) a gene encoding an outer membrane protein (omp-2) (JPF/JPR). Some modifications on the reported techniques were applied during the present work to improve the outcome. Results showed that B4/B5 primer pair had the highest sensitivity for detection of positive samples (98%), JPF/JPR primer pair detected 88.4% of positive samples while F4/R2 primer pair was the least sensitive being able to detect only 53.1% of positive samples. The specificity of the 3 techniques was 100%. B4/B5 primers were also able to detect the smallest number of bacteria (700 cfu/ml) while JPF/JPR were able to detect 7x105 cfu/ml and F4/R2 primers were able to detect 7x107 cfu/ml. It is thus concluded that using the B4/B5 primer PCR with the suggested modifications is a robust assay, which meets the sensitivity requirements to be used for testing of human blood samples for brucellosis in the diagnostic laboratory.