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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11236

Title: Evolutionary relationship and species separation of four morphologically similar stichotrichous ciliates (Protozoa, Ciliophora).
Authors: Clamp, J.,
AL-Rasheid, K. A. S.,
Song, W.
Chen, Z.
Keywords: Ciliophora;
Pseudokeronopsis;
Apokeronopsis
PCR-RFLP
ITS2 secondary structures
تاريخ النشر: 2009
Publisher: Progress in Natural Sciences. CHINA.
Citation: 19: 581-58.
Abstract: Phylogenetic and taxonomic studies on ciliate protists using molecular approaches have been demonstrated to be very reliable to formstrong conclusions and results. In the present work, species separation of some morphologically similar stichotrichous ciliates, two speciesof Pseudokeronopsis and two species of Apokeronopsis, was reexamined using amplified ribosomal DNA restriction analysis (PCRRFLP).Five of 10 restriction enzymes revealed species-specific polymorphic patterns, of which four similar stichotrichs could be significantlyseparated and identified. Among them, EcoR I offered almost no significantly different restriction fragment patterns, but the fourspecies could be separated from one another and identified with Hae III. Distinctly different restriction digestion haplotypes and similarityindices separated the species, and were used to construct a phylogeny. Phylogenies based on ITS2 nucleotide sequences and ITS2secondary structures supported the separation of Pseudokeronopsis and Apokeronopsis using RFLP analysis, although three Pseudokeronopsiscarnea populations did not cluster together. In addition, phylogenetic analyses using multiple algorithms confirmed that thesetwo genera formed two distinct groups within the urostylids. 2008 National Natural Science Foundation of China and Chinese Academy of Sciences.:
URI: http://hdl.handle.net/123456789/11236
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