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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11274

Title: Effect of DNA Hypomethylation on Genotoxicity and Apoptogenicity of Sodium arsenite in laboratory Mice.
Authors: Saad Alkahtani
F. M. Abou.Tarboush
Ahmed Al-Qahtani.
Keywords: Methylation
Sodium arsenite
Lamina-A. Mice. 5-Aza.C
Issue Date: 2009
Publisher: Pakistan Journal of Biological Sciences
Citation: 12(7): 554-564
Abstract: Living organisms are expose to a wide variety of natural or induced environmental pollutants such as arsenic compounds which can be reach to human body by different ways. The present study aims to evaluate the effect of DNA hypomethylation state on genotoxicity and apoptogenicity induced by sodium arsenite (NaAsO2) in normal adult male SWR/J mouse bone marrow cells. Animals were treated with intraperitoneally (ip) injected with (2.25, 4.50 or 9 mg/kg B.W of NaAsO2 which represent 0.25, 0.50 or 1 of LD5 respectively), and killed 24 hours later. Another different groups of male mice were treated with three doses of 5-azacitidine (5-AzaC), 5 mg/kg B.W. each dose and three hours intervals between them. NaAsO2 administered after 6 days of the last dose. The three single doses of sodium arsenite alone significantly (P<0.05) increased the rate of total structural chromosomal aberrations (CAs), rate of sister chromatid exchanges (SCEs), micronucleus (MNs) formation, PARP and Lamia-A degradation, and apoptosis as compared with the negative control. The comined treatment with hypomethylation agent 5-AzaC significantly increased the rate of SCEs induced by NaAsO2 at low dose. Moreover this treatment significantly increased the rate of polyploidy at all combined used doses. Furthermore, this treatment induced apoptosis at all used doses. The present study has shown that DNA hypomethylation had a negative effects represented in rate of (CAs), polyploidy, PARP degradation and apoptosis induced by (NaAsO2). On the other hand, DNA hypomethylation had a positive effects represented in decreased the rate of pulverized chromosomes, centromeric attenuations, (SCEs), (MNs) formation, prevent Lamina-A degradation and apoptosis.
URI: http://hdl.handle.net/123456789/11274
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