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Title: In vitro and in vivo production of pectolytic enzymes by some phytopathogenic fungi isolated from southwest Saudi Arabia
Authors: Bahkali, A.H.
Al-Khaliel, A.S.
Elkhider, K.A.
Keywords: Vitro and Vivo Production
Pectolytic Enzymes
Phytopathogenic Fungi
Issue Date: 1997
Publisher: King Saud University
Citation: Journal of king Saud University, Science: 9(2); 125-137
Abstract: Five fungal plant pathogens isolated from crops grown in southwest Saudi Arabia were investigated for their in vitro and in vivo ability to produce pectolytic enzymes. Only Alternaria macrospora did not produce any appreciable amount of enzymes in vivo and in vitro. Pythium debaryanum, Fusarium oxysporum, Rhizoctonia solani, Fusarium equiseti produced pectolytic enzymes in vitro. These same four fungi produced pectolytic enzymes in vivo which were detected after ten-fold concentration of blended infected host tissue. The pH values of peak enzyme activity of 4.0 and 5.0 indicate that the enzymes produced were polygalacturonases. The monogalacturonide end-product of pectin and cell wall degradation indicate that exopolygalacturonases were produced by the pathogens. High levels of in vitro<br>endopolygalacturonaes, determined viscometrically, and the detection oflow concentration of these enzymes in infected host plant tissues, and also the slow pectin-splitting action of the exopolygalacturonases produced, may account for the rot development and the wilt nature of the diseases these four fungi cause.
URI: http://hdl.handle.net/123456789/1128
Appears in Collections:Journal of the King Saud University - Science

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