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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11334

Title: Evaluation of protein and lipid composition of plasma membrane of Zea mays L. in response to relative to copper and cadmium tolerance
Authors: Mohamed M. Ibrahim
Adel Abd El-Aal
Rehab M. El-Dakak
Keywords: Heavy metals;
plasma membrane
protein; fatty acids
Zea mays
Issue Date: 2011
Publisher: Elsevier
Abstract: The effect of copper and cadmium on the modification of protein and plasma membrane(P.M.) in maize leaves and roots was studied. In stressed plants for 4 d with 0.25. 1 and 4 mM Cu or Cd the amount of total lipid in the plasma membranes of leaves and roots were decreased. Also, the total sterols in P.M. of the treated roots were decreased whereas, that of the leaves were slightly increased (insignificant) or unchanged in response to heavy metals used. On the other hand, the treatment had no significant effect on total glycolipids. Furthermore, the direct effects of Cu2+ and Cd2+ treatments on the lipid composition of membranes by altering the profile of unsaturated fatty acids have been reported,. After 4 days exposure to Cu2+ and Cd2+ over 50% of the total fatty acids were saturated in plasma membrane of both leaves and roots of Cu2+ and Cd2+ treated plant. SDS-PAGE analysis of protein pattern showed some polypeptide bands were disappeared under Cu2+ and Cd2+ treatments but at different concentrations. The presence of various protein bands which differ in migration position and bands’ intensities, proving the significant effect of both Cu2+ and Cd2+ on the protein content of leaves through induction of new stress protein bands. Taken together, these data strongly indicated that alteration of the protein and plasma membrane lipid composition under the effect of Cu2+ and Cd2+ stresses were mainly due to the reduction in lipid and protein set synthesized during the experimental period or by partial protein and lipid degradation and this is the initial cause of growth reduction under metals treatment in short-term experiments.
URI: http://hdl.handle.net/123456789/11334
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