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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/17117

Title: A highly sensitive enzyme immunoassay for evaluation of 2'-deoxycytidine plasma level as a prognostic marker for breast cancer chemotherapy.
Authors: Ibrahim A Darwish
Ashraf M Mahmoud
Tarek Aboul-Fadl
Abdul-Rahman A Al-Majed
Nasr Y Khalil
Keywords: Antibodies, Monoclonal, Biological Markers, Blood Chemical Analysis, Breast Neoplasms, Cattle, Cell Line, Tumor, Chromatography, High Pressure Liquid, Deoxycytidine, Immunoenzyme Techniques, Mice, Prognosis, Rats, Reproducibility of Results, Sensitivity and Specificity, Serum Albumin, Bovine, blood, metabolism, methods, drug therapy
Issue Date: 2009
Publisher: An Chim Acta
Abstract: A highly sensitive competitive enzyme immunoassay (EIA) has been developed and validated for the determination of the plasma level of 2'-deoxycytidine (dCyd), the potential prognostic marker for breast cancer chemotherapy. This assay employed a monoclonal antibody that recognizes dCyd with a high specificity, and 5'-succinyl-dCyd (5'sdCyd) conjugate of bovine serum albumin (5'sdCyd-BSA) immobilized onto microplate wells as a solid phase. The assay involved a competitive binding reaction between dCyd, in plasma sample, and the immobilized 5'sdCyd-BSA for the binding sites of the anti-dCyd antibody. The bound antibody was quantified with horseradish peroxidase-labeled anti-immunoglobulin second antibody and 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate. The concentration of dCyd in the sample was quantified by its ability to inhibit the binding of the antibody to the immobilized 5'sdCyd-BSA and subsequently the color formation in the assay. The assay limit of detection was 8 nM and the effective working range at relative standard deviations (R.S.D.s).
URI: http://hdl.handle.net/123456789/17117
Appears in Collections:College of Pharmacy

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