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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/17156

Title: Coupling of TLC and UV-measurement for quantification of naproxen and its main metabolite in urine.
Authors: E M Abdel-Moety
A M Al-Obaid
E A Lotfi
Issue Date: 1989
Publisher: Eur J Drug Metab Pharmacokin
Abstract: A simple sensitive method of high specificity and selectivity for quantitative determination of the non-steroidal anti-inflammatory drug naproxen and its main metabolite, 6-demethylated derivative, in biological specimens is described. Like naproxen, its metabolite absorbs maximally at 232 nm; this makes their simultaneous quantification, via direct UV-measurements at lambda max, in biological fluids quite impossible. Simple TLC-separation on silica gel F254 using chloroform + methanol (85:15, v/v) achieved the best fractionation of the unchanged drug and its metabolite from the matrix-contents of urine. UV-quantification of fractionated components could reach concentration levels of 0.2-3.0 micrograms ml-1 (ppm) in worked up urine samples. Varying levels of unchanged antiinflammatory drug and the phenolic metabolite could be accurately traced in urine samples following a 2.9 mg/kg oral dose after different time-intervals. Synthetic preparation of the metabolite by demethylation of naproxen is briefly mentioned.
URI: http://hdl.handle.net/123456789/17156
Appears in Collections:College of Pharmacy

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