HPLC quantification of azintamide and papaverine hydrochloride simultaneously in pharmaceutical preparations.

Abstract

Twenty tablets were powdered, and a portion containing ≃10 mg of azintamide (I) and ≃1 mg of papaverine hydrochloride (II) was extracted by mechanical shaking for ≃15 min with 80 ml of mobile phase [acetonitrile - H2O (14:11)]. The vol. was adjusted to 100 ml with mobile phase, the mixture was filtered, and portions of the filtrate were diluted to 20 ml before analysis on a stainless-steel column (12.5 cm × 4 mm) of Hibar 100RP-18 LiChrospher (5 µm) with a mobile phase flow rate of 1 ml min–1, and 240-nm detection. The recoveries of I and II were 100.39 ± 0.70% (n = 12) and 99.97 ± 1.11% (n = 12), respectively. The method was accurate, with coeff. of variation (n = 10) of 0.7% and 0.9%, respectively, for the analysis of standard mixtures containing 5.5 and 0.55 µg ml–1.