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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/17234

Title: Enantioselective high-performance liquid chromatographic method for the determination of baclofen in human plasma.
Authors: Mohamed M Hefnawy
Hassan Y Aboul-Enein
Issue Date: 2003
Publisher: Talenta
Abstract: A new analytical method for the separation and determination of R-(-)- and S-(+)- baclofen enantiomers in human plasma by high-performance liquid chromatography (HPLC) with UV detection was developed. Enantioselective resolution of the baclofen enantiomers was achieved by using teicoplanin macrocyclic antibiotic chiral stationary phase (CSP) known as Chirobiotic T with a polar ionic mobile phase (PIM) consisting of methanol: glacial acetic acid: triethylamine, 100:0.1:0.1, (v/v/v) at a flow rate of 0.5 ml min(-1) and UV detection set at 220 nm. The analytes of interest with S-(+)-sulpiride as the internal standard were extracted from human plasma using liquid-liquid extraction procedure with ethyl ether under alkaline condition prior to HPLC analysis. Recoveries for R-(-)- and S-(+)-baclofen enantiomers were in the ranges of 96-103% at 60-2500 ng ml(-1) level. Intra-day and inter-day precision calculated as %RSD was in the ranges of 1.2-5.2 and 1.3-4.3% for both enantiomers, respectively. Intra-day and inter-day accuracy calculated as percentage error were in the ranges of 1.2-3.9 and 1.1-3.9% for both enantiomers, respectively. Linear calibration curves in the concentration ranges of 20-3000 ng ml(-1) for each enantiomer showed correlation coefficient (r) of 0.9997. The limit of quantitation (LOQ) and limit of detection
URI: http://hdl.handle.net/123456789/17234
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