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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/18517

Title: ROLE OF INTERMEDIARY BIOMARKERS IN DETERMINING THE ANTICANCER EFFICACY OF MARINE COMPOUNDS
Authors: M. Arif, Jamal.
Kunhi, Mohammed.
M. Siddiqui, Yunus.
A. Sayed, Khalid.
Y. Orabi, Khaled.
Al-Hazzani, Amal.
N. Al-Ahdal, Mohammed.
M. Al-Khodairy, Fahad.
Issue Date: 2004
Publisher: SPRINGERLINK MEDICINAL CHEMISTRY RESEARCH Volume 13
Abstract: In the present study, two of the probable an umor marine compounds, manzamine A and sarcophine, were screened using benzo[a]pyrene (BP)-derived DNA adduct formation in MCF-7 cells as intermediary biomarker. Briefly, MCF-7 cells were treated with the compounds for 24 h followed by treatment with BP (0.5 μM). After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique. Manzamine A and sarcophine increased the BP-DNA adducts by 2 to 4-folds. Further, manzamine A (50 μM) substantially down regulated the expression of p53 while sarcophine (50 μM) slightly induced the level of p21. The residual DNA repair ability was almost completely abolished by manzamine A while sarcophine was ineffective. Based on our preliminary results, these compounds may be classified as potential genotoxic.
URI: http://hdl.handle.net/123456789/18517
Appears in Collections:College of Science

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