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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/18553

Title: 1. Cytochrome P450 1A as a Biomarker of Benzo-a-Pyrene Pollution in
Authors: May Joma, Nasser.
Virk, Promy.
A. Elobeid, Mai.
Keywords: Biomarker, Benzo-a-pyrene, cytochrome P450 1A, EROD activity, Freshwater fish
Issue Date: 2011
Publisher: Cogent SciTech
Citation: International Journal of Environmental Science and Engineering Research Vol 2(1): 15-22
Abstract: Induction of ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P450 1A (CYP1A) expression was used as a biomarker to assess the effect of waterborne exposure to Benzo-a- pyrene (B-a-P) Oreochromis niloticus and Pangasias sutchi. Both species were separately exposed, to three doses of B-a-P (0.01, 0.1 and 1 mg/L) at six different exposure times (6, 12, 24, 48, 72 and 96 hours). Enzyme activity and protein content were measured fluorimetrically in the hepatic S9 fraction. The response appeared as early as 6 hours post exposure. Both dose and time dependant response was observed in the EROD activity, being significantly higher at 48 hours for Oreochromis niloticus and at 12 hours for Pangasias sutchi, post exposure to 1mg /L. A similar response was observed in the CYP1A content in Pangasias sutchi, however, only a dose dependant response was detected in Oreochromis niloticus. Both groups showed higher values of EROD activity as compared to the CYP1A content, being higher in Pangasias sutchi. Our results show that hepatic CYP1A expression in terms of induction of EROD activity shows promise as a biomarker of organic contamination in the aquatic environment. Nevertheless, the work could be extended to assess the species specific response.
URI: http://hdl.handle.net/123456789/18553
ISSN: 0976-3708
Appears in Collections:College of Science

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