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|Title: ||Isolation of antimicrobial peptides from Apis florae|
|Authors: ||Hassan Ayaad, Tahany.|
Hamed Shaker, Ghada.
Muhnaa Almuhnaa, Amal.
|Keywords: ||Antimicrobial peptides;Honey;Apis florae;Apis carnica;Antimicrobial properties|
|Issue Date: ||2011 |
|Citation: ||King Saud University Journal of King Saud University Science www.ksu.edu.sa www.sciencedirect.com|
|Abstract: ||As part of the ongoing search for novel antimicrobial agents and their use in singular or
combined drug therapy, peptide fractions of molecular weights about 14.500 and 15.00 kDa were
isolated from the hemolymph of wild (Apis florae) and carniolan (Apis carnica) bees of Saudi Arabia
obtained from different regions in Riyadh (variable plant sources) during the honey seasons (spring
and summer) 2008–2009. Following experimental infection with 1.1 • l06 viable Escherichia coli cells
(ATCC 25922), the antimicrobial peptides were purified to homogeneity by reversed-phase high
performance liquid chromatography. Antibacterial activity of the isolated peptide was evaluated
in vitro by an agar well diffusion method for E. coli strain (ATCC 25922) and Klebsiella pneumoniae
strain (ATCC 11678), the major Gram negative pathogens causing urinary tract infections, and
Staphylococcus aureus (ATCC 6538) as Gram positive bacteria. A total of 10 honey samples collected
from bee hives selected arbitrary at different floral areas of south Riyadh were also investigated
for their antimicrobial activities against the yeast, Candida albicans (ATCC 10231) and four
standard bacteria strains, E. coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), S. aureus
(ATCC 6538) and Bacillus subtilis (ATCC 6633) using standard antimicrobial assays. The isolated
antibacterial peptides and the different honey samples revealed comparable marked variations in
antimicrobial activities and their sensitivity might be depending on their variable floral sources.
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|Appears in Collections:||College of Science|
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