Fluorimetric determination of isoxsuprine hydrochloride inpharmaceuticals and biological fluids

Abstract

Two simple and highly sensitive fluorimetric methods have been developed for the determination of isoxsuprinehydrochloride in bulk, in dosage forms and in biological fluids. The first method involves the direct measurement ofthe native fluorescence of the drug in the concentration range 0.4–4.0µg ml−1, the second method is based on theoxidation of isoxsuprineHCl with cerium(IV) followed by fluorimetric measurement in the concentration range0.02–0.2µg ml−1. The average % found were 99.9± 0.78 and 100.0± 0.62 for the two methods, respectively. Theminimum detectability (3 SB) were 0.11 and 0.007 µg ml−1 for the two methods, respectively. The methods resultsshowed insignificant difference with those of the official method.