Spectrofluorimetric Determination of Paroxetine Hydrochloride in ItsFormulations and Human Plasma

Abstract

A sensitive spectrofluorimetric procedure for the determination of paroxetine-HCl in pharmaceutical formulationsand human plasma has been described. The native fluorescence of the drug has been studied underdifferent conditions. Maximum fluorescence intensity was obtained in methanol at 340 nm using 290 nm for excitation.Different surfactants showed negative effect on the fluorescence intensity of paroxetine-HCl. Regressionanalysis of Beer’s plot showed good correlation (r = 0.9999) between fluorescence intensity and concentration overthe range of 0.05-0.40µgml-1 with lower limit of detection (LOD) of 0.015µgml-1. The drug was successfullydetermined in its tablets with average % recovery of 98.00-0.99% which was in accordance with those given bya compendial method. The method was also applied to the determination of paroxetine-HCl in spiked humanplasma with average recovery of 77.70±1.06%.