Sensitive assay for carvedilol in tablets and spiked human plasma using a flow-injection chemiluminometric method

Abstract

A simple and sensitive chemiluminometric method using flow injection (FI) is developed for the determination of carvedilol, based on the reaction of carvedilol with tris (2,2’-bipyridyl)ruthenium (II), and KMnO4 in a sulfuric acid medium. Under the optimum conditions; the chemiluminescence (CL) intensity is a linear function of carvedilol concentration over the range of 0.04–1.0 gml-1 (9.810-8 – 2.510-6 molL 1) with a detection limit (3noise) of 0.025 gml-1 (6.210-8 molL-1). The relative standard deviation of the proposed method calculated from 10 replicate injections of 0.4 g ml-1 carvedilol is 0.95%. The sample throughput is 90 samples h-1. The method is applied successfully to the determination of carvedilol in tablets dosage form and spiked human plasma.

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