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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/4435

Title: Comparison of mecA Polymerase Chain Reaction With Phenotypic Methods for the Detection of Methicillin-Resistant Staphylococcus aureus
Authors: Baddour, M.M.
AbuElKheir, M.M.
Fatani, A.J.
Keywords: methicillin-resistant
Staphylococcus aureus (MRSA)
Polymerase chain reaction (PCR)
Methicillin-resistant Staphylococcus aureus (MRSA)
Polymerase chain reaction (PCR)
Isolates in clinical settings.
Issue Date: 2007
Publisher: Springer
Citation: Curr Microbiol: 55; 473–479
Abstract: In the present study, several conventional methods to detect methicillin-resistant Staphylococcus aureus (MRSA) were compared with polymerase chain reaction (PCR) detection of mecA gene–positive isolates. Cefoxitin E-test was also evaluated as a possible phenotypic method of MRSA detection. Oxacillin agar screen and PBP2′ latex agglutination methods were found to be more sensitive than oxacillin and cefoxitin disk-diffusion methods. Cefoxitin disk diffusion was found to be the most specific. A combination of oxacillin agar screening with cefoxitin disk diffusion, or oxacillin disk diffusion with PBP2′, improved sensitivity and specificity. Cefoxitin E-test with the current break points had low sensitivity and specificity (33.3% and 75%, respectively) for the detection of MRSA. However, changing the break points to ≤ 4 μg/ml and to ≥ 6 μg/ml for sensitive and resistant, respectively, greatly improved both. Changing the 30-μg cefoxitin disk-diffusion break points to ≤ 21 mm for resistant slightly improved sensitivity but had no effect on specificity. It was therefore concluded that the use of more than one screening method is necessary to detect all MRSA isolates in clinical settings.
Description: Author: AbuElKheir M. M. Microbiology Department, King Saud University, P. O. Box 22452, Riyadh 11495, Saudi Arabia Author: Fatani, A.J. Pharmacology Department, King Saud University, P. O. Box 616, Riyadh 11421, Saudi Arabia
URI: http://hdl.handle.net/123456789/4435
Appears in Collections:College of Pharmacy

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