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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/490

Title: Extraction of proteolytic enzymes from pancreatic glands of Riyadh slaughter houses animal
Authors: AI-Shammary, Fahad Jaber
Keywords: Proteolytic Enzymes
Pancreatic Glands
Slaughter houses
Medical Sciences
Veterinary Sciences
Issue Date: 1993
Publisher: King Saud University
Citation: Journal of King Saud University, Science: 5 (1); 3-10
Abstract: Pancreatic glands of different animals such as cattle, goat and sheep were used for the preparation of acetone dried powders of trypsin and chymotrypsin. The soluble fractions of enzymes were isolated from the minced pancreas with sodium chloride, precipitated with ammonium sulphate, dialysed and dried. The activities of the enzymes were determined using synthetic substrates, N-benzoyl-L-arginine-ethyl- ester hydrochloride for trypsin and N-acetyl-L-tyrosine ethyl ester for chymotrypsin. Maximum trypsin recovery in acetone dried poser obtained from I g of pancreatic tissue was found to be 135.1 units in ox. As regards the soluble fraction, cow pancreas produced maximum enzyme yield with a total activity of 106.28 units per/g of pancreatic tissue. Similarly maximum chymotrypsin recovery in acetone dried powder obtained from 1 g of pancreatic tissue was found to be 166.52 units in ox. As regards the soluble fractions cow pancreas yielded maximum enzyme activity of 93.38 units per g of pancreatic tissue.
URI: http://hdl.handle.net/123456789/490
Appears in Collections:Journal of the King Saud University - Science

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