Sensitive assay for carvedilol in tablets and spiked human plasma using a flow-injection chemiluminometric method

Abstract

A simple and sensitive chemiluminometric method using flow injection (FI) is developed for the determination of carvedilol, based on the reaction of carvedilol with tris (2, 2'-bipyridyl) ruthenium (II), and KMnO 4 in sulfuric acid medium. Under the optimum conditions; the chemiluminescence (CL) intensity is a linear function of carvedilol concentration over the range of 0.04-1.0 Jig ml-1 (9.8 x 10-8 - 2.5 x 10' mol L -I) with a detection limit (S/N=3) of 0.025 Jlgml-1 (6.2 x 10-8 mol L -I). The relative standard deviation of the proposed method calculated from 10 replicate injections of 0.4 Jig ml-1 carvedilol is 0.95%. The sample throughput is 90 samples h-i. The method is applied successfully to the determination of carvedilol in tablets dosage form and spiked human plasma