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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/5489

Title: A possible modulatory role of nitric oxide in paraquat-induced lung Injury in mice
Authors: Gamal el-Din, Ayman M
Al-Majed, Abdulhakeem A
Al-Yahya, Abdulaziz A
Al-Bekairi, Abdullah M
Mostafa, Adel M
Keywords: Paraquat
L-arginine
L-NAME
Aminoguanidine
Nitric oxide
Lung-toxicity
Issue Date: 2005
Publisher: Asian Network of Scientific Information
Citation: International Journal of Pharmacology: 1 (4); 360-365
Abstract: The present study was undertaken to evaluate whether stimulation or inhibition of Nitric Oxide (NO) synthesis could affect lung toxicity induced by acute administration of paraquat (PQ) in mice. L-arginine (L-arg.), NG-nitro-L-arginine methyl ester (L-NAME) and aminoguanidine (AG) were employed as NO precursor, competitive and specific NO synthesis inhibitors, respectively. PQ was administered intraperitoneally to Swiss albino mice at a single dose of 50 mg kg- 1 . L-arg. (700 mg k g - 1 day- 1), L-NAME (150 mg kg- 1 day- 1 ) or AG (100 mg k g - 1 day- 1 ) was given in drinking water of mice for 5 days before and one day after PQ administration. Appropriate controls were performed. PQ administration resulted in a pronounced elevation in lipid peroxides (157%) as well as decreased activity of alkaline phosphatase [ALP] (48%) and non-protein thiols (40%) in lung tissue compared to control non-treated mice as evidences of lung injury. Serum level of NO end products, nitrate and nitrite significantly elevated due to PQ administration (150%) as compared to control level. In mice given combined treatment of L-arg. and PQ, a remarkable rise in the serum level of nitrate and nitrite (140%) compared to the PQ group was observed. In addition, L-arg. ameliorated the increased level of lipid peroxides and non-protein thiols depletion as well as the decreased activity of ALP caused by PQ respectively. On the other hand, L-NAME and AG potentiated the deleterious effects of PQ on serum NO, lung lipid peroxides content, non-protein thiols content and alkaline phosphatase activity. In conclusion, PQ-induced lung injury in mice is alleviated by L-arg. but exacerbated by L-NAME and AG supplementation. This could point out to a possible protective role of NO in PQ lung toxicity.
URI: http://hdl.handle.net/123456789/5489
ISSN: 1811-7775
Appears in Collections:College of Pharmacy

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