The effect of 51Cr-labelling cell Morphology, in vitro, when evaluating the Cytotoxicity of endodontic filling materials

Abstract

Human periodontal ligament fibroblasts and L 929 cell line labelled with chromium 51 were examined by electron microscope to evaluate the effect of the chromium labeling on the cell ultrastructure. The cells were labeled with chromium 12-20 hours before the start of the experiment. After two and four hours of incubation at 37°C and 100% humidity, the cells were examined by scanning and transmission electron microscopy. The result showed that the chromium labeling did not cause any morphological changes.