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|Title: ||Microbial and metal analysis of some patients hair and nails of Alamal Hospital in Riyadh|
|Authors: ||AL Qahtani, Saeed yahya Mohi|
|Keywords: ||Microbial and Metal Analysis|
Hair and Nails
|Issue Date: ||2004 |
|Abstract: ||This study endeavor to detect presence of microorganisms (bacteria and/or fungi) in
hair and nails of some patients hosted in Alamal Hospital , Riyadh , Saudi Arabia, that
may become opportunist pathogens at somewhat favourable condition. Sixty-four
random swabs from end of shaft of scalp hair and nails sample were taken from
smoking and drug abusers patient men.
Seven bacterial genera and ten fungal genera have been isolated from the tested
samples, but nails contains higher bacteria than hair and hair contains higher fungi than
nails. The predominant bacteria found were Staphylococcus sp., while Aspergillus sp.
was the most fungi isolated.
Also, Sixty-four random samples of scalp hair and nails were obtained from smoking
and drug abusers patient men. All samples collected were analysed for determining the
present of heavy metals in the body. The analytical study may cover some toxic heavy
metal such as cadmium, cobalt, copper, iron, lead and zinc . Also the study aim to figure
out the role of alcohol, smoking and some drug addicts in increasing or decreasing the
residue content of the above mentioned metals. Hair and nails can act as accumulating
tissue, so, they are good indicators for body burden of heavy metals for along period.
The results provide evidence of high accumulation of heavy metals in hair and nails, it
showed that, cadmium, cobalt, copper, iron, lead and zinc are heavy metals detected in
this analysis. The average of metal concentration detected are (Cd: 1.14 ± 0.04, 1.53 ±
0.07; Co: 4.92 ± 0.24, 3.20 ± 0.05; Cu: 19.22 ± 0.53, 13.09 ± 0.54; Fe: 20.72 ± 0.49,
23.93 ± 0.2 ; Pb: 24.20 ± 0.43, 28.58 ± 0.75; Zn: 63.01 ± 1.44, 40.38 ± 1.57) μgg-1 in
the hair and nails respectively.
Statistical analysis (T) test indicates that the concentration of Cd, Co, Pb and Zn
were significantly higher in hair of patients compared to healthy person at (P ≤ 0.05),
while the concentration of Fe and Cu were not significantly different at (P = 0.05).The
concentration levels of the (Cd, Co, Cu, Fe, Pb and Zn) in the nails of the candidate
participants. Statistical analysis shows that the concentration of Cd, Cu, Pb and Zn were
significantly higher in nails of the patients than those of healthy person at (P ≤ 0.05),
however, there was no significant difference in concentration levels of Co and Fe
between patients and healthy people at (P = 0.05).
The accumulation levels of cobalt, copper and zinc were significantly higher in the
hair of the patients that their nails (P ≤ 0.05), although this study revealed significant
different in heavy metal contents of cadmium, cobalt, copper, iron, lead and zinc in hair
and nails, the study support the result of earlier work, however still no work has been
reported to explain this accumulative of heavy metal in hair and nails.
The present study investigate the ability of some microorganisms isolated from hair and
nails samples of Al-amal hospital patients to tolerate the toxicity of a multi heavy metal
medium and then possible application in the remediation of metal pollution.
The effect of cadmium, cobalt, and iron on the growth of fungal strains (Candida
albicans, Microsporum cains and Aspergillus nigar) respectively were examined. Also,
The effect of copper, lead and zinc on the growth of bacterial strains (Pseudomonas
aeruginosa, Staphylococcus epidermidis and Escherichia coli) respectively were
examined. The results showed that Candida albicans, Microsporum cains and
Aspergillus nigar can grow in media containing up to (300) μgml-1 of cadmium , (200)
μgml-1 of cobalt and (400) μgml-1 of iron respectively, and Pseudomonas aeruginosa,
Staphylococcus epidermidis and Escherichia coli can grow in media containing up to
(400) μgml-1 of copper, iron and zinc respectively.|
|Description: ||A thesis submitted in Partial Fulfillment of Master Degree of Science
|Appears in Collections:||Deanship of Higher Education|
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