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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/9022

Title: M M Alfuraiji', P J Broadbent', J S M Hutchinson 1,2, D F Dolman1, T Atkinson2 1School of Agriculture
Authors: Aberdeen AJf9 IUD
تاريخ النشر: 1989
Abstract: The aim of this study was to examine the effect of a monoclonal antibody to pregnant mare serum gonadotropin (PMSG) (Neutra-P!1SG; Intervet UK) on embryo yield and quality when used in parous beef x dairy (suckler) cows. Seventy-two Hereford x British Friesian cows were assigned at random to one of four treat¬ment groups. Estrus was synchronized using two injections of prostaglandin (PG) as 2 ml Prosol vin (Intervet UK) given 11 days apart. Animals were super¬ovulated with 2500 iu PMSG (Fa lligon; Intervet UK) im on day 10 of their estrous cycle and given 2 ml PG im 48 h later. Cows were inseminated twice with two straws of semen 12 and 24 h after the onset of estrus. In addition, cows received 5 ml of the monoclonal antibody via the jugular vein 36, 48 and 60 h after the PG injection in Groups II, III and IV, respectively. Controls (Group I) were given the same quantity of placebo (anti-PMSG vehicle) and six cows were so treated at each time of administration. Embryos were collected nonsurgically on day 6 to 8 of the cycle and numbers of corpora lutea (CL) and cystic follicles (CF) for each cow were counted by palpation. Plasma estradiol (E2) levels were measured by radioimmunoassay. Data were analyzed by ANOVA and means were compared by LSD. Circulating ~ in superovulated cows always showed a peak value 24 or 48 h after administration of PG. In the control cows ~ did not return to basal levels after the peak but showed a second increase after estrus, then decreased slowly to basal levels. In all cows treated with anti¬PMSG, however, E2 declined to basal levels within 24 h after treatment and remained low until flushing. The anti-PHSG treatment at 48 and 60 h after PG injection also reduced (P<O.05) CF numbers compared to those of the control group. Anti-PMSG given at 36, 48 or 60 h after PG injection, however, did not significantly affect the ovulation rate, the total number of ova/embryos col¬lected or the number of usable embryos (Table).
URI: http://hdl.handle.net/123456789/9022
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