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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/9023

Authors: M M Alfuraiji
P J Broadbent
Issue Date: 1990
Abstract: The object of the experiment was to examine the effects of mono¬clonal anti-PMSG (Neutra-PMSG; Intervet UK), the dose level of pregnant mare serum gonadotropin (PMSG), and the timing of administering anti-PMSG on embryo yield and guality. Sixty-four Hereford X British Friesian heifers were assigned at random to one of thirty-two treatment groups in a 2 X 4 X 4 factorial design. The factors were PMSG or PMSG plus anti-PMSG (PMSG/APMSG) PMSG administered 4 dose levels and APMSG injected at 4 times after prostaglandin (PG) injection. Estrus was synchronized using two injections of PG as 2 ml Prosolvin (Intervet UK) given 11 days apart. Heifers were superovulated with 1000, 2000, 3000 or 4000 iu of PMSG (Folligon; Intervet UK) im on day 10±1 of their estrous cycle and given 2 ml PG im 48 h later. Animals were inseminated twice with two straws of semen 12 and 30 h after the onset of estrus. An equivalent dosage of PMSG-antiserurn to the PMSG dosage (PMSG/APMSG group) or the anti-PMSG vehicle (PMSG group) was administered iv to heifers 60, 72, 84 or 96 h after PG injection. Embryos were collected nonsurgically on day 6 to 8 of the superovulated estrous cycle and numbers of corpora lutea (CL) and large follicles (LF) >10rnrn in diameter were counted visually by laparoscopy on the following day. Plasma estradiol (E2) was measured by radioimmunoassay. Data were analyzed by ANOVA and means were compared by LSD. Preovulatory peak concentration of E2 occurred 24 to 48 h after PG injection. In the PMSG group a second peak of E2 occurred 5 days after estrus which was followed by a slow decline to basal levels. In the PMSG/APMSG group E2 dropped to basal levels within 24 h after the injection of APMSG and remained low until the last day of the experiment. Overall, the numbers of CL, LF, total number of ova/embryos and the number of usable embryos were not significantly different (P>0.05) between PMSG and PMSG/APMSG group (8.5, 3.4, 5.7 and 4. 1 v 9.0, 1.5, 5.0 and 4.1, respectively). The numbers of LF were, however, 2.3 times higher in PMSG than PMSG/APMSG treatment. When data were evaluated based on the time of APMSG administration, administering APMSG 60, 84 or 96 h after PG reduced significantly (P<0.05) the numbers of LF as compared to PMSG groups (2.3, 1.9 and 0.6 v 3.4, 5.3 and 2.1, respectively). When data were evaluated in respect of the PMSG/APMSG dose level, there were no significant differences (P>0.05) in the numbers of CL and usable number of embryos between APMSG treatment and the appropriate control dose level. Treatment with APMSG in the 3000 iu PMSG dose group reduced (P<0.05) the numbers of LF compared to control (1.3 v 5.5).In conclusion, despite reducing LF numbers and preventing the rise in E2 after ovulation, Neutra-PMSG had no significant effect on the number of usable embryos recovered.
URI: http://hdl.handle.net/123456789/9023
Appears in Collections:College of Foods And Agricultural Science

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