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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/9032

Title: The Zoonotic Source of Brucellosis in Saudi Arabia
Issue Date: 2005
Abstract: Background: Brucellosis is a major zoonotic disease caused by gram-negative bacteria of the genus Brucella. The disease is presented with protean manifestations that pose a serious public Hlth. and economic problem worldwide. In Saudi Arabia, the disease is endemic in rural areas but the actual reservoir of its zoonotic insult has not been determined. This study was conducted to determine the animal source of human brucellosis in farms with recurrent episodes of brucellosis. Materials and Methods: One hundred sera were collected from Brucella -infected and non-vaccinated sheep, cattle, camels, and humans for the detection of Brucella DNA by PCR as previously described (Elfaki et a/ .,2004). Negative sera for brucellosis were evaluated similarly and used as negative controls. Results: PCR results showed that a genus-specific amplicon of 223-bp was detected in 87 % (20/23) of camel serum DNA samples. In contrast, serum DNA samples tested similarly by the PCR method showed positive Brucella DNA amplicons of 223-bp in 74 (26/35) and 68 (15/22) % of sheep and cattle, respectively. In humans that were occupationally exposed to these animals, 65 % (13/20) of positive Brucella DNA amplicons of 223-bp were detected. No band was detected when the primers were used to amplify DNA derived from Brucella -negative sera. Conclusions: These results suggest that farm animals are implicated in the transmission of brucellosis and that camels are the major reservoir for human infection in Saudi Arabia. It is evident that brucellosis would remain a major zoonotic threat in the Arabian Peninsula.
URI: http://hdl.handle.net/123456789/9032
Appears in Collections:College of Foods And Agricultural Science

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