Toxicity and Biochemical Effects of Lead, Cadmium, Acetamiprid and Their Mixtures on Male Mice

Abstract

The toxicity and biochemical effects of lead ( 10 mg / kg / day ), cadmium ( 1 mg/kg/day ), acetamiprid ( 34 & 63 mg/kg/day ) and their mixtures after 14 days of oral administration on male white albino mice were investigated. Lead and cadmium treatments caused high percent mortalities 55.6 % and 66.7 % respectively, while acetamiprid at 67.9 mg/kg/day caused 11.1 % only. The mixtures of acetamiprid with lead and cadmium decreased the percent mortalities of lead and cadmium, this means that acetamiprid ( cyanoimin group ) had an antagonistic effect to heavy metals. Furthermore, we studied the effect of lead and cadmium treatments on activity of -ALAD, AChE and GST enzymes. Both caused significantly decrease of the activity of -ALAD ( 52.12 % & 19.94 % ), AChE ( 42.57 % & 28 % ) and GST ( 14.37 % & 34.32 % ), while they caused elevation of serum LDH level ( 40 % & 46.76 % ), respectively. On the other hand, the mixtures of acetamiprid with lead and cadmium decreased the inhibitory effect of lead and cadmium on -ALAD & AChE activity and inhibited the elevation of serum LDH level. A correlation between toxicity and changes of enzyme activities as a result of lead, cadmium treatment and their mixtures with acetamiprid, was quite clear and demonstrated that acetamiprid may acts as a protective agent against heavy metals intoxication .