Effects of Treated Municipal Waste Water on Meloidogyne javanica Egg Hatch and Penetration

Abstract

Eggs of Meloidogyne javanica were exposed in vitro to increasing concentrations of treated municipal waste water ( TMWW ) over a period of 8 days. Hatched juveniles ( J2 ) were extracted and counted every 2 days. Juveniles that hatched from day 6 to day 8 were based as inoculum to determine their capability to invade tomato roots under greenhouse conditions. TMWW suppressed egg hatch at all concentrations, at least 4 days after incubation. After day 4 the hatch suppression was proportional to TMWW concentrations, or incubation period. Hatch suppressions ( % of control ) at day 8 were 11.3, 27.8, 34.4 and 51 % at concentrations of 25, 50, 75, and 100 % TMWW, respectively. Invasion of tomato roots by the hatched juveniles was not adversely affected.