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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/9762

Title: Optimization of Extracellular Inulinase Production of Kluyveromyces marxianus Strain NRRL 2415
Authors: Wael A. Bazaraa
Mosffer M. Al-Dagal
Keywords: Inulin, K. marxianus, Inulinase, Fructose, Fructan-Fructano hydrolase, Optimization
Issue Date: 1999
Abstract: Production of extracellular inulinase (fructan fructanohydrolase) E.C. by Kluyveromyces marxianus NARL 2415 in batch shaking system was optimized. Inulinase from K. marxianus NRRL 2415 was found to be constitutive; whereas the enzyme of K. marxianus ATCC 8601 was of the inducible types. Several factors were tested for its effect on enzyme synthesis by K. marxianus NRRL e.g., carbon source concentration, pH of the growth media, nitrogen source, nitrogen concentration and incubation temperature. The highest inulinae production (10670 U/L) was achieved when test organism (NRRL 2415) was allowed to grow aerobically in buffered medium (pH 7.0) containing 4% sucrose, 2% yeast extract and 0.6% sodium dihydrogen phosphate at 30º. After optimization, enzyme activity as well as productivity increased by a factor of 4.6, each.
URI: http://hdl.handle.net/123456789/9762
Appears in Collections:College of Foods And Agricultural Science

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