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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/9914

Title: Efficacy of Pseudomonas to Phytoremediate Nickel by Canola (Brassica napus L.)
Authors: Ashour, Eman H
R. A. El-Mergawi
S. M. A. Radwan
Issue Date: 2006
Publisher: Journal of Applied Sciences Research
Abstract: The use of plants for rehabilitation of heavy metal-contaminated environments is an emerging area of interest because it provides an ecologically sound and safe method for restoration and remediation. Although a number of plant species are capable of hyperaccumulation of heavy metals, the technology is not applicable for remediating sites with contaminates. A good solution is to combine the advantages of microbe-plant symbiosis within the plant rhizosphere into an effective cleanup technology. Four Pseudomonas strains, as plant growth promoting rhizobacteria, were examined for their ability to grow in synthetic medium supplemented with nickel. Minimal inhibitory concentration for Pseudomonas strains was displayed 3 mM Ni. Canola germination was evaluated in the presence of four Pseudomonas strains and different Ni concentrations (0, 0.5, 1.0 and 1.5 mM). Germination percent, length and fresh weight of seedling significantly decreased by increasing Ni in germination media. A positive response of bacteria inoculations was observed forward the toxic effect of Ni on germination percent, especially in the presence of high Ni level . P. putida strain stimulated the seedling elongation in Ni-free culture. Bacterial inoculation increased fresh weight of seedlings under 1 mM of Ni, relative to corresponded uninoculated seedlings. In pot experiments, data indicated that the presence of Ni in irrigation water significantly promoted canola biomass. Whereas, enhancement of canola growth as a response of Pseudomonas inoculants was not significant. The beneficial of bacteria on plant growth varied depending on individual bacterial strain. Presence of Ni at 2 mM, produced plants containing more than tenfold of Ni relative to zero Ni plants. Tendency of Pseudomonas-treated plants to accumulate more Ni relative to uninoculated was observed, especially for high Ni-treated plants. The accumulation of Ni/plant increased about 60% in P. fluorescens inoculated canola more than uninoculated plants under high Ni level (2 mM). Higher accumulation of Ni in Pseudomonas-treated plants was attributed mainly to alteration in Ni uptake more than to Pseudomonas effect on plant growth. These results have been suggested to use canola inoculated with P. fluorescens as a promising strategy to remediate metal-contaminated sites.
URI: http://hdl.handle.net/123456789/9914
Appears in Collections:College of Foods And Agricultural Science

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